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Lifetime computing algorithms based on exponential pattern retrieve and polynomial fitting in fluorescence lifetime imaging microscopy
Liu, Chao; Zhou, Yan; Wang, Xinwei; Liu, Yuliang; Liu, C.
2011
Source PublicationProceedings of SPIE- The International Society for Optical Engineering
ISSN0277786X
Volume8200Pages:82000X
AbstractHow to simulate the decay pattern is crucial during lifetime inversion while utilizing intensity images acquired at increasing delays in time gated fluorescence lifetime imaging microscopy(FLIM) method. A relatively novel understanding of fluorescence decay pattern theory and stimulation algorithms of time gated FLIM method have been analyzed in this paper comprehensively. Main lifetime computing algorithms can be classified as exponential pattern retrieve and polynomial fitting procedure. Especially, a novel lifetime computing method based on bi-exponential decay has been discussed. In experiment, we have validated the proposed algorithms utilizing synthetic images. Performances like calculating precision and computing speed of the algorithms above have also been compared.?2011 SPIE CCC code.
metadata_83光电系统实验室
KeywordFluorescence Numerical Analysis Optical Instruments Technology
Subject Area光电子学
Indexed ByEI
Language英语
Date Available2012-06-14
Document Type期刊论文
Identifierhttp://ir.semi.ac.cn/handle/172111/23088
Collection光电系统实验室
Corresponding AuthorLiu, C.
Recommended Citation
GB/T 7714
Liu, Chao,Zhou, Yan,Wang, Xinwei,et al. Lifetime computing algorithms based on exponential pattern retrieve and polynomial fitting in fluorescence lifetime imaging microscopy[J]. Proceedings of SPIE- The International Society for Optical Engineering,2011,8200:82000X.
APA Liu, Chao,Zhou, Yan,Wang, Xinwei,Liu, Yuliang,&Liu, C..(2011).Lifetime computing algorithms based on exponential pattern retrieve and polynomial fitting in fluorescence lifetime imaging microscopy.Proceedings of SPIE- The International Society for Optical Engineering,8200,82000X.
MLA Liu, Chao,et al."Lifetime computing algorithms based on exponential pattern retrieve and polynomial fitting in fluorescence lifetime imaging microscopy".Proceedings of SPIE- The International Society for Optical Engineering 8200(2011):82000X.
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