| Partial transfection of cells using laminar flows in microchannels | |
| Li L; Nie Y; Shi XT; Wu HK; Ye DT; Chen HD; Wu, HK (reprint author), Tohoku Univ, WPI Adv Inst Mat Res, Aoba Ku, 2-1-1 Katahira, Sendai, Miyagi 9808577, Japanchhkwu@ust.hk; yedt6386@sz.tsinghua.edu.cn | |
| 2011 | |
| Source Publication | BIOMICROFLUIDICS
 |
| ISSN | 1932-1058 |
| Volume | 5Issue:3Pages:36503 |
| Abstract | This manuscript describes a convenient method for partial transfection using a Y-shaped microchannel polydimethylsiloxane (PDMS)-glass chip and on-chip cationic lipid-mediated transfection. Enhanced green fluorescent protein genes (pEGFP-N2) were introduced into the COS-7 cells cultured in half of the channel, while red fluorescent protein genes (pDsRed-N1) were introduced into the cells cultured in another half of the channel. This on-chip partial transfection technique provides an avenue for the spatial control of transfection. It is possible to use this technique to perform parallel transfection on chips in order to study cell behaviors under two or more gene transfections in the same culture. (C) 2011 American Institute of Physics. [doi: 10.1063/1.3643827] |
| metadata_83 | 集成光电子学国家重点实验室 |
| Keyword | Mammalian-cells Chip Electroporation Chemotaxis |
| Subject Area | 光电子学 |
| Funding Organization | Shenzhen Bureau of Science, Technology and Information[041201005]; China Postdoctoral Science Foundation[20090460498]; National Natural Science Foundation of China[61078074]; National Basic Research Program of China[2011CB933100, 2011CB933200] |
| Indexed By | SCI |
| Language | 英语 |
| Date Available | 2012-02-06 |
| Document Type | 期刊论文 |
| Identifier | http://ir.semi.ac.cn/handle/172111/22853 |
| Collection | 集成光电子学国家重点实验室 |
| Corresponding Author | Wu, HK (reprint author), Tohoku Univ, WPI Adv Inst Mat Res, Aoba Ku, 2-1-1 Katahira, Sendai, Miyagi 9808577, Japanchhkwu@ust.hk; yedt6386@sz.tsinghua.edu.cn |
| Recommended Citation GB/T 7714 | Li L,Nie Y,Shi XT,et al. Partial transfection of cells using laminar flows in microchannels[J]. BIOMICROFLUIDICS,2011,5(3):36503. |
| APA | Li L.,Nie Y.,Shi XT.,Wu HK.,Ye DT.,...&yedt6386@sz.tsinghua.edu.cn.(2011).Partial transfection of cells using laminar flows in microchannels.BIOMICROFLUIDICS,5(3),36503. |
| MLA | Li L,et al."Partial transfection of cells using laminar flows in microchannels".BIOMICROFLUIDICS 5.3(2011):36503. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| Partial transfection（1635KB） | 限制开放 | -- | Application Full Text | |||
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