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Partial transfection of cells using laminar flows in microchannels
Li L; Nie Y; Shi XT; Wu HK; Ye DT; Chen HD; Wu, HK (reprint author), Tohoku Univ, WPI Adv Inst Mat Res, Aoba Ku, 2-1-1 Katahira, Sendai, Miyagi 9808577, Japanchhkwu@ust.hk; yedt6386@sz.tsinghua.edu.cn
2011
Source PublicationBIOMICROFLUIDICS
ISSN1932-1058
Volume5Issue:3Pages:36503
AbstractThis manuscript describes a convenient method for partial transfection using a Y-shaped microchannel polydimethylsiloxane (PDMS)-glass chip and on-chip cationic lipid-mediated transfection. Enhanced green fluorescent protein genes (pEGFP-N2) were introduced into the COS-7 cells cultured in half of the channel, while red fluorescent protein genes (pDsRed-N1) were introduced into the cells cultured in another half of the channel. This on-chip partial transfection technique provides an avenue for the spatial control of transfection. It is possible to use this technique to perform parallel transfection on chips in order to study cell behaviors under two or more gene transfections in the same culture. (C) 2011 American Institute of Physics. [doi: 10.1063/1.3643827]
metadata_83集成光电子学国家重点实验室
KeywordMammalian-cells Chip Electroporation Chemotaxis
Subject Area光电子学
Funding OrganizationShenzhen Bureau of Science, Technology and Information[041201005]; China Postdoctoral Science Foundation[20090460498]; National Natural Science Foundation of China[61078074]; National Basic Research Program of China[2011CB933100, 2011CB933200]
Indexed BySCI
Language英语
Date Available2012-02-06
Document Type期刊论文
Identifierhttp://ir.semi.ac.cn/handle/172111/22853
Collection集成光电子学国家重点实验室
Corresponding AuthorWu, HK (reprint author), Tohoku Univ, WPI Adv Inst Mat Res, Aoba Ku, 2-1-1 Katahira, Sendai, Miyagi 9808577, Japanchhkwu@ust.hk; yedt6386@sz.tsinghua.edu.cn
Recommended Citation
GB/T 7714
Li L,Nie Y,Shi XT,et al. Partial transfection of cells using laminar flows in microchannels[J]. BIOMICROFLUIDICS,2011,5(3):36503.
APA Li L.,Nie Y.,Shi XT.,Wu HK.,Ye DT.,...&yedt6386@sz.tsinghua.edu.cn.(2011).Partial transfection of cells using laminar flows in microchannels.BIOMICROFLUIDICS,5(3),36503.
MLA Li L,et al."Partial transfection of cells using laminar flows in microchannels".BIOMICROFLUIDICS 5.3(2011):36503.
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